A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children.

ABSTRACT:

Background

Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death.

Methodology

Among 63 confirmed dengue pediatric patients recruited, hospital based longitudinal study detected six SD-SPL and ten dengue with warning sign (DWS). To identify the specific proteins increased or decreased in the SD-SPL plasma obtained 6-48 hours before the shock compared with the DWS, the isobaric tags for relative and absolute quantification (iTRAQ) technology was performed using four patients each group. Validation was undertaken in 6 SD-SPL and 10 DWS patients.

Principal findings

Nineteen plasma proteins exhibited signi

SUBMITTER: Nhi DM 

PROVIDER: S-EPMC4764501 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature