Mass spectrometry data from proteomic analysis of human skin keratins after exposure to UV radiation.

ABSTRACT: A mass spectrometry (MS)-based proteomic methodology was employed to monitor oxidative modifications in keratins, the main constituents of human skin ("Non-invasive proteomic analysis of human skin keratins: screening of methionine oxidation in keratins by mass spectrometry" [1], "UV irradiation-induced methionine oxidation in human skin keratins: mass spectrometry-based non-invasive proteomic analysis" [2]). Human skin proteins were obtained non-invasively by tape stripping and solubilized in sodium dodecyl sulfate (SDS) buffer, followed by purification and digestion using the filter-aided sample preparation method. The tryptic peptides were then analyzed by liquid chromatography (LC)/electrospray ionization (ESI)-MS, tandem MS (MS/MS), and LC/ESI-selected reaction monitoring (SRM)/MS. Th

SUBMITTER: Lee SH 

PROVIDER: S-EPMC4764772 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature