Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization.

ABSTRACT: Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application of genetic immunization to a structurally diverse set of membrane proteins to show that immunization of mice with DNA alone produced antibodies against 71% (n = 17) of the bacterial and viral targets. Antibody production correlated with prior reports of target immunogenicity in host organisms, underscoring the efficiency of this DNA-gold micronanoplex approach. To generate each antigen for antibody characterization, we also developed a simple in vitro membrane protein expression and capture method. Antibody specificity was demonstrated upon identifying, for the fi

SUBMITTER: Hansen DT 

PROVIDER: S-EPMC4764931 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature