Evaluation of different nested PCRs for detection of Anaplasma phagocytophilum in ruminants and ticks.

ABSTRACT:

Background

Anaplasma phagocytophilum is a causative agent of granulocytic anaplasmosis in mammals, which has a broad geographical distribution and a high degree of clinical diversity. Currently, numerous PCR assays have been developed and used for the detection of A. phagocytophilum in various specimens. However, their performance varies. The aim of this study was to evaluate the performance of five nested PCR assays by detection of 363 ruminant and tick samples, and to select the most appropriate methods for the sensitive detection of A. phagocytophilum in environmental or clinical samples.

Results

Positive PCR results for A. phagocytophilum were obtained in 75 (20.7%), 42 (11.6%) and 19 (5.2%) specimens with primer sets EC (EC9/EC12a and SSAP2f/SSAP2r), EE (EE1/EE2 and EE3

SUBMITTER: Yang J 

PROVIDER: S-EPMC4765105 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature