Gelam honey potentiates ex vivo corneal keratocytes proliferation with desirable phenotype expression.

ABSTRACT:

Background

This study aimed to evaluate the effects of Gelam honey on corneal keratocytes proliferative capacity and phenotypic characterization via MTT assay, gene expression and immunocytochemistry.

Methods

Corneal keratocytes from New Zealand white rabbits were cultured in basal medium (BM) and serum enriched medium (BMS). Serial dilutions of Gelam honey (GH) were added to both media and cells were cultured until passage 1. MTT assay was performed on corneal keratocytes in both media to ascertain the optimal dose of GH that produced maximum proliferation.

Results

Gelam honey at the concentration of 0.0015% in both media showed the highest proliferative capacity with no morphological changes compared to their respective controls. The gene expression of aldehyde dehy

SUBMITTER: Yusof AM 

PROVIDER: S-EPMC4765115 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature