Autoantibodies against the Second Extracellular Loop of M3R Do neither Induce nor Indicate Primary Sjogren's Syndrome.

ABSTRACT:

Objectives

Anti-muscarinic acetylcholine type-3 receptor (anti-M3R) autoantibodies have been suggested to be pathogenic for primary Sjögren's syndrome (pSS), and the second extracellular loop of M3R is suspected to carry a disease-promoting epitope. In this study, we aimed to evaluate the pathogenicity of autoantibodies against peptides derived from the second extracellular loop of M3R in mice and to determine whether those autoantibodies could be used as biomarker for pSS.

Methods

BALB/c mice were immunized with modified linear or cyclic peptides of the second extracellular loop of M3R. The function of exocrine glands was evaluated by measuring the secretion of saliva and tears. The histological evaluations were performed by using H&E staining or direct immunofluorescence s

SUBMITTER: Chen Y 

PROVIDER: S-EPMC4765836 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature