A generic methodological framework for studying single cell motility in high-throughput time-lapse data.

ABSTRACT:

Motivation

Motility is a fundamental cellular attribute, which plays a major part in processes ranging from embryonic development to metastasis. Traditionally, single cell motility is often studied by live cell imaging. Yet, such studies were so far limited to low throughput. To systematically study cell motility at a large scale, we need robust methods to quantify cell trajectories in live cell imaging data.

Results

The primary contribution of this article is to present Motility study Integrated Workflow (MotIW), a generic workflow for the study of single cell motility in high-throughput time-lapse screening data. It is composed of cell tracking, cell trajectory mapping to an original feature space and hit detection according to a new statistical procedure. We show that thi

SUBMITTER: Schoenauer Sebag A 

PROVIDER: S-EPMC4765885 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature