Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.

ABSTRACT:

Background & aims

Human intestinal crypt-derived enteroids are a model of intestinal ion transport that require validation by comparison with cell culture and animal models. We used human small intestinal enteroids to study neutral Na(+) absorption and stimulated fluid and anion secretion under basal and regulated conditions in undifferentiated and differentiated cultures to show their functional relevance to ion transport physiology and pathophysiology.

Methods

Human intestinal tissue specimens were obtained from an endoscopic biopsy or surgical resections performed at Johns Hopkins Hospital. Crypts were isolated, enteroids were propagated in culture, induced to undergo differentiation, and transduced with lentiviral vectors. Crypt markers, surface cell enzymes, and membran

SUBMITTER: Foulke-Abel J 

PROVIDER: S-EPMC4766025 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature