Profiling the genome-wide DNA methylation pattern of porcine ovaries using reduced representation bisulfite sequencing.

ABSTRACT: Substantial evidence has shown that DNA methylation regulates the initiation of ovarian and sexual maturation. Here, we investigated the genome-wide profile of DNA methylation in porcine ovaries at single-base resolution using reduced representation bisulfite sequencing. The biological variation was minimal among the three ovarian replicates. We found hypermethylation frequently occurred in regions with low gene abundance, while hypomethylation in regions with high gene abundance. The DNA methylation around transcriptional start sites was negatively correlated with their own CpG content. Additionally, the methylation level in the bodies of genes was higher than that in their 5' and 3' flanking regions. The DNA methylation pattern of the low CpG content promoter genes differed obviously fro

SUBMITTER: Yuan XL 

PROVIDER: S-EPMC4766444 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature