Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children.

ABSTRACT: No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested fo

SUBMITTER: Mortensen R 

PROVIDER: S-EPMC4766568 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature