Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementing culture media with copper(II).

ABSTRACT:

Background

The ability to site-specifically conjugate a protein to a payload of interest (e.g., a fluorophore, small molecule pharmacophore, oligonucleotide, or other protein) has found widespread application in basic research and drug development. For example, antibody-drug conjugates represent a class of biotherapeutics that couple the targeting specificity of an antibody with the chemotherapeutic potency of a small molecule drug. While first generation antibody-drug conjugates (ADCs) used random conjugation approaches, next-generation ADCs are employing site-specific conjugation. A facile way to generate site-specific protein conjugates is via the aldehyde tag technology, where a five amino acid consensus sequence (CXPXR) is genetically encoded into the protein of interest at th

SUBMITTER: York D 

PROVIDER: S-EPMC4766608 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature