Mapping and candidate gene screening of tomato Cladosporium fulvum-resistant gene Cf-19, based on high-throughput sequencing technology.

ABSTRACT:

Background

Tomato leaf mold is a common disease in tomato cultivation. This disease is caused by Cladosporium fulvum, which has many physiological races and differentiates rapidly. Cf genes confer resistance to C. fulvum, and the C. fulvum-tomato pathosystem is a model for the study of gene-for-gene interactions. Plants carrying the Cf-19 gene show effective resistance to C. fulvum in the field, and can be used in breeding and resistance mechanism studies as new resistant materials. In this study, we used F2 bulk specific-locus amplified fragment sequencing (SLAF-seq) and parental resequencing methods to locate and characterize the Cf-19 gene.

Results

A total of 4108 Diff_markers and three association regions were found in association analysis. A 2.14-Mb region containing se

SUBMITTER: Zhao T 

PROVIDER: S-EPMC4766677 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature