Cross-platform comparison of microarray data using order restricted inference.

ABSTRACT:

Motivation

Titration experiments measuring the gene expression from two different tissues, along with total RNA mixtures of the pure samples, are frequently used for quality evaluation of microarray technologies. Such a design implies that the true mRNA expression of each gene, is either constant or follows a monotonic trend between the mixtures, applying itself to the use of order restricted inference procedures. Exploiting only the postulated monotonicity of titration designs, we propose three statistical analysis methods for the validation of high-throughput genetic data and corresponding preprocessing techniques.

Results

Our methods allow for inference of accuracy, repeatability and cross-platform agreement, with minimal required assumptions regarding the underlying data

SUBMITTER: Klinglmueller F 

PROVIDER: S-EPMC4766743 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature