Characterization and relative quantification of phospholipids based on methylation and stable isotopic labeling.

ABSTRACT: Phospholipids (PLs), one of the lipid categories, are not only the primary building blocks of cellular membranes, but also can be split to produce products that function as second messengers in signal transduction and play a pivotal role in numerous cellular processes, including cell growth, survival, and motility. Here, we present an integrated novel method that combines a fast and robust TMS-diazomethane-based phosphate derivatization and isotopic labeling strategy, which enables simultaneous profiling and relative quantification of PLs from biological samples. Our results showed that phosphate methylation allows fast and sensitive identification of the six major PL classes, including their lysophospholipid counterparts, under positive ionization mode. The isotopic labeling of endogenous

SUBMITTER: Cai T 

PROVIDER: S-EPMC4766988 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature