Comparative genomic and genetic analysis of glioblastoma-derived brain tumor-initiating cells and their parent tumors.

ABSTRACT:

Background

Glioblastoma (GBM) is a fatal cancer that has eluded major therapeutic advances. Failure to make progress may reflect the absence of a human GBM model that could be used to test compounds for anti-GBM activity. In this respect, the development of brain tumor-initiating cell (BTIC) cultures is a step forward because BTICs appear to capture the molecular diversity of GBM better than traditional glioma cell lines. Here, we perform a comparative genomic and genetic analysis of BTICs and their parent tumors as preliminary evaluation of the BTIC model.

Methods

We assessed single nucleotide polymorphisms (SNPs), genome-wide copy number variations (CNVs), gene expression patterns, and molecular subtypes of 11 established BTIC lines and matched parent tumors.

Results

SUBMITTER: Davis B 

PROVIDER: S-EPMC4767234 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature