An informatic framework for decoding protein complexes by top-down mass spectrometry.

ABSTRACT: Efforts to map the human protein interactome have resulted in information about thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we report a computational search strategy that supports hierarchical top-down analysis for precise identification and scoring of multi-proteoform complexes by native mass spectrometry.

SUBMITTER: Skinner OS 

PROVIDER: S-EPMC4767540 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature