RNA-sequencing study of peripheral blood monocytes in chronic periodontitis.

ABSTRACT:

Background

Monocytes are an important cell type in chronic periodontitis (CP) by interacting with oral bacteria and mediating host immune response. The aim of this study was to reveal new functional genes and pathways for CP at monocyte transcriptomic level.

Methods

We performed an RNA-sequencing (RNA-seq) study of peripheral blood monocytes (PBMs) in 5 non-smoking moderate to severe CP (case) individuals vs. 5 controls. We took advantage of a microarray study of periodontitis to support our findings. We also performed pathway-based analysis on the identified differentially expressed (DEx) transcripts/isoforms using DAVID (Database for Annotation, Visualization and Integrated Discovery).

Results

Through differential expression analyses at both whole gene (or whole non

SUBMITTER: Liu YZ 

PROVIDER: S-EPMC4767619 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature