Epigenetic alteration to activate Bmp2-Smad signaling in Raf-induced senescence.

ABSTRACT:

Aim

To investigate epigenomic and gene expression alterations during cellular senescence induced by oncogenic Raf.

Methods

Cellular senescence was induced into mouse embryonic fibroblasts (MEFs) by infecting retrovirus to express oncogenic Raf (RafV600E). RNA was collected from RafV600E cells as well as MEFs without infection and MEFs with mock infection, and a genome-wide gene expression analysis was performed using microarray. The epigenomic status for active H3K4me3 and repressive H3K27me3 histone marks was analyzed by chromatin immunoprecipitation-sequencing for RafV600E cells on day 7 and for MEFs without infection. These data for Raf-induced senescence were compared with data for Ras-induced senescence that were obtained in our previous study. Gene knockdown and overex

SUBMITTER: Fujimoto M 

PROVIDER: S-EPMC4768123 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature