Quantifying Lgr5-positive stem cell behaviour in the pyloric epithelium.

ABSTRACT: Using in-vivo lineage tracing data we quantified clonal expansion as well as proliferation and differentiation of the Lgr5-positive stem cell population in pyloric gastric glands. Fitting clone expansion models, we estimated that there are five effective Lgr5-positive cells able to give rise to monoclonal glands by replacing each other following a pattern of neutral drift dynamics. This analysis is instrumental to assess stem cell performance; however, stem cell proliferation is not quantified by clone expansion analysis. We identified a suitable mathematical model to quantify proliferation and differentiation of the Lgr5-positive population. As expected for populations in steady-state, the proliferation rate of the Lgr5-positive population was equal to its rate of differentiation. This ra

SUBMITTER: Leushacke M 

PROVIDER: S-EPMC4768140 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature