In-situ and real-time growth observation of high-quality protein crystals under quasi-microgravity on earth.

ABSTRACT: Precise protein structure determination provides significant information on life science research, although high-quality crystals are not easily obtained. We developed a system for producing high-quality protein crystals with high throughput. Using this system, gravity-controlled crystallization are made possible by a magnetic microgravity environment. In addition, in-situ and real-time observation and time-lapse imaging of crystal growth are feasible for over 200 solution samples independently. In this paper, we also report results of crystallization experiments for two protein samples. Crystals grown in the system exhibited magnetic orientation and showed higher and more homogeneous quality compared with the control crystals. The structural analysis reveals that making use of the magneti

SUBMITTER: Nakamura A 

PROVIDER: S-EPMC4768258 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature