Simple perfusion apparatus for manipulation, tracking, and study of oocytes and embryos.

ABSTRACT:

Objective

To develop and implement a device and protocol for oocyte analysis at a single cell level. The device must be capable of high resolution imaging, temperature control, perfusion of media, drugs, sperm, and immunolabeling reagents all at defined flow rates. Each oocyte and resultant embryo must remain spatially separated and defined.

Design

Experimental laboratory study.

Setting

University and academic center for reproductive medicine.

Patient(s)/animal(s)

Women with eggs retrieved for intracytoplasmic sperm injection (ICSI) cycles, adult female FVBN and B6C3F1 mouse strains, sea stars.

Intervention(s)

Real-time, longitudinal imaging of oocytes after fluorescent labeling, insemination, and viability tests.

Main outcome measure(s)

Cell and

SUBMITTER: Angione SL 

PROVIDER: S-EPMC4768872 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature