Most highly expressed protein-coding genes have a single dominant isoform.

ABSTRACT: Although eukaryotic cells express a wide range of alternatively spliced transcripts, it is not clear whether genes tend to express a range of transcripts simultaneously across cells, or produce dominant isoforms in a manner that is either tissue-specific or regardless of tissue. To date, large-scale investigations into the pattern of transcript expression across distinct tissues have produced contradictory results. Here, we attempt to determine whether genes express a dominant splice variant at the protein level. We interrogate peptides from eight large-scale human proteomics experiments and databases and find that there is a single dominant protein isoform, irrespective of tissue or cell type, for the vast majority of the protein-coding genes in these experiments, in partial agreement wit

SUBMITTER: Ezkurdia I 

PROVIDER: S-EPMC4768900 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature