Topographical effects on fiber-mediated microRNA delivery to control oligodendroglial precursor cells development.

ABSTRACT: Effective remyelination in the central nervous system (CNS) facilitates the reversal of disability in patients with demyelinating diseases such as multiple sclerosis. Unfortunately until now, effective strategies of controlling oligodendrocyte (OL) differentiation and maturation remain limited. It is well known that topographical and biochemical signals play crucial roles in modulating cell fate commitment. Therefore, in this study, we explored the combined effects of scaffold topography and sustained gene silencing on oligodendroglial precursor cell (OPC) development. Specifically, microRNAs (miRs) were incorporated onto electrospun polycaprolactone (PCL) fiber scaffolds with different fiber diameters and orientations. Regardless of fiber diameter and orientation, efficient knockdown of d

SUBMITTER: Diao HJ 

PROVIDER: S-EPMC4769075 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature