GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler.

ABSTRACT:

Background

Transcription factor-dependent cellular reprogramming is integral to normal development and is central to production of induced pluripotent stem cells. This process typically requires pioneer transcription factors (TFs) to induce de novo formation of enhancers at previously closed chromatin. Mechanistic information on this process is currently sparse.

Results

Here we explore the mechanistic basis by which GATA3 functions as a pioneer TF in a cellular reprogramming event relevant to breast cancer, the mesenchymal to epithelial transition (MET). In some instances, GATA3 binds previously inaccessible chromatin, characterized by stable, positioned nucleosomes where it induces nucleosome eviction, alters local histone modifications, and remodels local chromatin archite

SUBMITTER: Takaku M 

PROVIDER: S-EPMC4769547 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature