Control of alternative end joining by the chromatin remodeler p400 ATPase.

ABSTRACT: Repair of DNA double-strand breaks occurs in a chromatin context that needs to be modified and remodeled to allow suitable access to the different DNA repair machineries. Of particular importance for the maintenance of genetic stability is the tight control of error-prone pathways, such as the alternative End Joining pathway. Here, we show that the chromatin remodeler p400 ATPase is a brake to the use of alternative End Joining. Using specific intracellular reporter susbstrates we observed that p400 depletion increases the frequency of alternative End Joining events, and generates large deletions following repair of double-strand breaks. This increase of alternative End Joining events is largely dependent on CtIP-mediated resection, indicating that it is probably related to the role of p40

SUBMITTER: Taty-Taty GC 

PROVIDER: S-EPMC4770216 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature