Altered DNA methylation of glycolytic and lipogenic genes in liver from obese and type 2 diabetic patients.

ABSTRACT:

Objective

Epigenetic modifications contribute to the etiology of type 2 diabetes.

Method

We performed genome-wide methylome and transcriptome analysis in liver from severely obese men with or without type 2 diabetes and non-obese men to discover aberrant pathways underlying the development of insulin resistance. Results were validated by pyrosequencing.

Result

We identified hypomethylation of genes involved in hepatic glycolysis and insulin resistance, concomitant with increased mRNA expression and protein levels. Pyrosequencing revealed the CpG-site within ATF-motifs was hypomethylated in four of these genes in liver of severely obese non-diabetic and type 2 diabetic patients, suggesting epigenetic regulation of transcription by altered ATF-DNA binding.

Conclusion

SUBMITTER: Kirchner H 

PROVIDER: S-EPMC4770265 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature