MiRepress: modelling gene expression regulation by microRNA with non-conventional binding sites.

ABSTRACT: Some earlier studies have reported an alternative mode of microRNA-target interaction. We detected target regions within mRNA transcripts from AGO PAR-CLIP that did not contain any conventional microRNA seed pairing but only had non-conventional binding sites with microRNA 3' end. Our study from 7 set of data that measured global protein fold change after microRNA transfection pointed towards the association of target protein fold change with 6-mer and 7-mer target sites involving microRNA 3' end. We developed a model to predict the degree of microRNA target regulation in terms of protein fold changes from the number of different conventional and non-conventional target sites present in the target, and found significant correlation of its output with protein expression changes. We validate

SUBMITTER: Ghosal S 

PROVIDER: S-EPMC4770313 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature