Highly diverse nirK genes comprise two major clades that harbour ammonium-producing denitrifiers.

ABSTRACT:

Background

Copper dependent nitrite reductase, NirK, catalyses the key step in denitrification, i.e. nitrite reduction to nitric oxide. Distinct structural NirK classes and phylogenetic clades of NirK-type denitrifiers have previously been observed based on a limited set of NirK sequences, however, their environmental distribution or ecological strategies are currently unknown. In addition, environmental nirK-type denitrifiers are currently underestimated in PCR-dependent surveys due to primer coverage limitations that can be attributed to their broad taxonomic diversity and enormous nirK sequence divergence. Therefore, we revisited reported analyses on partial NirK sequences using a taxonomically diverse, full-length NirK sequence dataset.

Results

Division of NirK sequences

SUBMITTER: Helen D 

PROVIDER: S-EPMC4770552 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature