NTRK1 rearrangement in colorectal cancer patients: evidence for actionable target using patient-derived tumor cell line.

ABSTRACT:

Background

We have investigated the incidence of NTRK1 rearrangements in metastatic gastrointestinal cancer patients and demonstrated the potential for clinical response of these patients to targeted therapy.

Methods

We prospectively collected tumor tissue specimens for one year and simultaneously generated patient-derived tumor cells (PDCs). Specimens were initially screened for TrkA protein expression using TrkA immunohistochemistry (IHC). In the case of TrkA IHC positive results, samples were further examined by fluorescence in situ hybridization (FISH) and next generation sequencing (NGS) to confirm the presence of NTRK1 rearrangements.

Results

From January 2014 to December 2014, a total of 74 metastatic colorectal cancer (CRC) patients and 66 gastric cancer (GC)

SUBMITTER: Lee SJ 

PROVIDER: S-EPMC4770754 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature