Bladder cancer cells re-educate TAMs through lactate shuttling in the microfluidic cancer microenvironment.

ABSTRACT:

Background

In the present study, we aimed to investigate the influence of lactate shuttling on the functional polarization and spatial distribution of transitional cell carcinoma of the bladder (TCCB) cells and macrophages.

Methods

We designed a microfluidic coculture chip for real-time integrative assays. The effect of lactate shuttling on the re-education of macrophages by TCCB cells was explored by measuring the levels of NO using a total NO assay kit and by evaluating the protein expression of iNOS, p-NFkB-p65, Arg-1 and HIF-1α via cell immunofluorescence and western blotting. Additionally, we examined TCCB cell viability using acridine orange/ethidium bromide (AO/EB) and MitoTracker staining. Moreover, the concentration distributions of lactate and large signaling prote

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC4770766 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature