Real-Time Monitoring of Cellular Bioenergetics with a Multianalyte Screen-Printed Electrode.

ABSTRACT: Real-time monitoring of changes to cellular bioenergetics can provide new insights into mechanisms of action for disease and toxicity. This work describes the development of a multianalyte screen-printed electrode for the detection of analytes central to cellular bioenergetics: glucose, lactate, oxygen, and pH. Platinum screen-printed electrodes were designed in-house and printed by Pine Research Instrumentation. Electrochemical plating techniques were used to form quasi-reference and pH electrodes. A Dimatix materials inkjet printer was used to deposit enzyme and polymer films to form sensors for glucose, lactate, and oxygen. These sensors were evaluated in bulk solution and microfluidic environments, and they were found to behave reproducibly and possess a lifetime of up to 6 weeks. Line

SUBMITTER: McKenzie JR 

PROVIDER: S-EPMC4770793 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature