Use of laser capture microdissection for the assessment of equine lamellar basal epithelial cell signalling in the early stages of laminitis.

ABSTRACT:

Reasons for performing study

Dysadhesion of laminar basal epithelial cells (LBECs) from the underlying dermis is the central event leading to structural failure in equine laminitis. Although many studies of sepsis-related laminitis have reported multiple events occurring throughout the lamellar tissue, there is minimal information regarding signalling events occurring specifically in LBECs.

Objectives

To determine signalling events in the LBECs during the early stages of carbohydrate-induced laminitis.

Study design

Experimental study.

Methods

Eight horses were given an overload of carbohydrate (CHO) consisting of corn starch mixture via nasogastric tube. Prior to administration of CHO, lamellar biopsies were taken from the left forefoot (control [CON]). Biopsie

SUBMITTER: Leise BS 

PROVIDER: S-EPMC4771185 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature