Two-photon imaging of chronically implanted neural electrodes: Sealing methods and new insights.

ABSTRACT:

Background

Two-photon microscopy has enabled the visualization of dynamic tissue changes to injury and disease in vivo. While this technique has provided powerful new information, in vivo two-photon chronic imaging around tethered cortical implants, such as microelectrodes or neural probes, present unique challenges.

New method

A number of strategies are described to prepare a cranial window to longitudinally observe the impact of neural probes on brain tissue and vasculature for up to 3 months.

Results

It was found that silastic sealants limit cell infiltration into the craniotomy, thereby limiting light scattering and preserving window clarity over time. In contrast, low concentration hydrogel sealants failed to prevent cell infiltration and their use at high concen

SUBMITTER: Kozai TD 

PROVIDER: S-EPMC4771525 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature