An optimized disaggregation method for human lung tumors that preserves the phenotype and function of the immune cells.

ABSTRACT: Careful preparation of human tissues is the cornerstone of obtaining accurate data in immunologic studies. Despite the essential importance of tissue processing in tumor immunology and clinical medicine, current methods of tissue disaggregation have not been rigorously tested for data fidelity. Thus, we critically evaluated the current techniques available in the literature that are used to prepare human lung tumors for immunologic studies. We discovered that these approaches are successful at digesting cellular attachments and ECMs; however, these methods frequently alter the immune cell composition and/or expression of surface molecules. We thus developed a novel approach to prepare human lung tumors for immunologic studies by combining gentle mechanical manipulation with an optimized co

SUBMITTER: Quatromoni JG 

PROVIDER: S-EPMC4771617 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature