Metabolic Trade-offs in Yeast are Caused by F1F0-ATP synthase.

ABSTRACT: Intermediary metabolism provides living cells with free energy and precursor metabolites required for synthesizing proteins, lipids, RNA and other cellular constituents, and it is highly conserved among living species. Only a fraction of cellular protein can, however, be allocated to enzymes of intermediary metabolism and consequently metabolic trade-offs may take place. One such trade-off, aerobic fermentation, occurs in both yeast (the Crabtree effect) and cancer cells (the Warburg effect) and has been a scientific challenge for decades. Here we show, using flux balance analysis combined with in vitro measured enzyme specific activities, that fermentation is more catalytically efficient than respiration, i.e. it produces more ATP per protein mass. And that the switch to fermentation at h

SUBMITTER: Nilsson A 

PROVIDER: S-EPMC4772093 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature