Overlapping cell population expression profiling and regulatory inference in C. elegans.

ABSTRACT:

Background

Understanding gene expression across the diverse metazoan cell types during development is critical to understanding their function and regulation. However, most cell types have not been assayed for expression genome-wide.

Results

We applied a novel approach we term "Profiling of Overlapping Populations of cells (POP-Seq)" to assay differential expression across all embryonic cells in the nematode Caenorhabditis elegans. In this approach, we use RNA-seq to define the transcriptome of diverse partially overlapping FACS-sorted cell populations. This identified thousands of transcripts differentially expressed across embryonic cells. Hierarchical clustering analysis identified over 100 sets of coexpressed genes corresponding to distinct patterns of cell type specific

SUBMITTER: Burdick J 

PROVIDER: S-EPMC4772325 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature