Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene.

ABSTRACT:

Background

Nested PCRs based on the Plasmodium 18s-rRNA gene have been extensively used for human malaria diagnosis. However, they are not practical when large quantities of samples need to be processed, further there have been challenges in the performance and when interpreting results, especially when submicroscopic infections are analysed. Here the use of "direct PCR" was investigated with the aim of improving diagnosis in the malaria elimination era.

Methods

The performance of the Plasmodium cytochrome oxidase III gene (COX-III) based novel malaria detection strategies (direct nested PCR and direct single PCR) were compared using a 18s-rRNA direct nested PCR as a reference tool. Evaluations were based on sensitivity, specificity and the ability to detect mixed infections

SUBMITTER: Echeverry DF 

PROVIDER: S-EPMC4772515 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature