Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli.

ABSTRACT:

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Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. Combined with cell divisions, this generates heterogeneous aggregate distributions in subsequent cell generations. We studied the robustness of this process with differing medium richness and antibiotics stress, which affect nucleoid size, using multimodal, time-lapse microscopy of live cells expressing both a fluorescently tagged chaperone (IbpA), which identifies in vivo the location of aggregates, and HupA-mCherry, a fluorescent variant of a nucleoid-associated protein. We find that the relative sizes of the nucleoid's major and minor axes change widely, in a positively correlated fashion, with medium richness and antibiotic stress. The aggregate's distribution along the major cell axis

SUBMITTER: Neeli-Venkata R 

PROVIDER: S-EPMC4772596 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature