A PTH-responsive circadian clock operates in ex vivo mouse femur fracture healing site.

ABSTRACT: The circadian clock contains clock genes including Bmal1 and Period2, and it maintains an interval rhythm of approximately 24 hours (the circadian rhythm) in various organs including growth plate and articular cartilage. As endochondral ossification is involved not only in growth plate but also in fracture healing, we investigated the circadian clock functions in fracture sites undergoing healing. Our fracture models using external fixation involved femurs of Period2::Luciferase knock-in mice which enables the monitoring of endogenous circadian clock state via bioluminescence. Organ culture was performed by collecting femurs, and fracture sites were observed using bioluminescence imaging systems. Clear bioluminescence rhythms of 24-hour intervals were revealed in fracture healing sites. Wh

SUBMITTER: Kunimoto T 

PROVIDER: S-EPMC4772627 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature