COLORFUL-Circuit: A Platform for Rapid Multigene Assembly, Delivery, and Expression in Plants.

ABSTRACT: Advancing basic and applied plant research requires the continuous innovative development of the available technology toolbox. Essential components of this toolbox are methods that simplify the assembly, delivery, and expression of multiple transgenes of interest. To allow simultaneous and directional multigene assembly on the same plant transformation vector, several strategies based on overlapping sequences or restriction enzymes have recently been developed. However, the assembly of homologous and repetitive DNA sequences can be inefficient and the frequent occurrence of target sequences recognized by commonly used restriction enzymes can be a limiting factor. Here, we noted that recognition sites for the restriction enzyme SfiI are rarely occurring in plant genomes. This fact was explo

SUBMITTER: Ghareeb H 

PROVIDER: S-EPMC4772762 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature