Synthetic RNA-protein modules integrated with native translation mechanisms to control gene expression in malaria parasites.

ABSTRACT: Synthetic posttranscriptional regulation of gene expression is important for understanding fundamental biology and programming new cellular processes in synthetic biology. Previous strategies for regulating translation in eukaryotes have focused on disrupting individual steps in translation, including initiation and mRNA cleavage. In emphasizing modularity and cross-organism functionality, these systems are designed to operate orthogonally to native control mechanisms. Here we introduce a broadly applicable strategy for robustly controlling protein translation by integrating synthetic translational control via a small-molecule-regulated RNA-protein module with native mechanisms that simultaneously regulate multiple facets of cellular RNA fate. We demonstrate that this strategy reduces 'lea

SUBMITTER: Ganesan SM 

PROVIDER: S-EPMC4773503 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature