Periscope: quantitative prediction of soluble protein expression in the periplasm of Escherichia coli.

ABSTRACT: Periplasmic expression of soluble proteins in Escherichia coli not only offers a much-simplified downstream purification process, but also enhances the probability of obtaining correctly folded and biologically active proteins. Different combinations of signal peptides and target proteins lead to different soluble protein expression levels, ranging from negligible to several grams per litre. Accurate algorithms for rational selection of promising candidates can serve as a powerful tool to complement with current trial-and-error approaches. Accordingly, proteomics studies can be conducted with greater efficiency and cost-effectiveness. Here, we developed a predictor with a two-stage architecture, to predict the real-valued expression level of target protein in the periplasm. The output of t

SUBMITTER: Chang CC 

PROVIDER: S-EPMC4773868 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature