Identification and analysis of murine pancreatic islet enhancers.

ABSTRACT:

Aims/hypothesis

The paucity of information on the epigenetic barriers that are blocking reprogramming protocols, and on what makes a beta cell unique, has hampered efforts to develop novel beta cell sources. Here, we aimed to identify enhancers in pancreatic islets, to understand their developmental ontologies, and to identify enhancers unique to islets to increase our understanding of islet-specific gene expression.

Methods

We combined H3K4me1-based nucleosome predictions with pancreatic and duodenal homeobox 1 (PDX1), neurogenic differentiation 1 (NEUROD1), v-Maf musculoaponeurotic fibrosarcoma oncogene family, protein A (MAFA) and forkhead box A2 (FOXA2) occupancy data to identify enhancers in mouse islets.

Results

We identified 22,223 putative enhancer loci in in

SUBMITTER: Tennant BR 

PROVIDER: S-EPMC4773896 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature