Characterization of an extensive rainbow trout miRNA transcriptome by next generation sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of gene expression in a wide variety of physiological processes. They can control both temporal and spatial gene expression and are believed to regulate 30 to 70% of the genes. Data are however limited for fish species, with only 9 out of the 30,000 fish species present in miRBase. The aim of the current study was to discover and characterize rainbow trout (Oncorhynchus mykiss) miRNAs in a large number of tissues using next-generation sequencing in order to provide an extensive repertoire of rainbow trout miRNAs.

Results

A total of 38 different samples corresponding to 16 different tissues or organs were individually sequenced and analyzed independently in order to identify a large number

SUBMITTER: Juanchich A 

PROVIDER: S-EPMC4774146 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature