Inhibition of protein kinase C promotes dengue virus replication.

ABSTRACT:

Background

Dengue virus (DENV) is a member of the Flaviviridae family, transmitted to human via mosquito. DENV infection is common in tropical areas and occasionally causes life-threatening symptoms. DENV contains a relatively short positive-stranded RNA genome, which encodes ten viral proteins. Thus, the viral life cycle is necessarily rely on or regulated by host factors.

Methods

In silico analyses in conjunction with in vitro kinase assay were used to study kinases that potentially phosphorylate DENV NS5. Potential kinase was inhibited or activated by a specific inhibitor (or siRNA), or an activator. Results of the inhibition and activation on viral entry/replication and host cell survival were examined.

Results

Our in silico analyses indicated that the non-structu

SUBMITTER: Noppakunmongkolchai W 

PROVIDER: S-EPMC4774189 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature