Detection and sequence analysis of accessory gene regulator genes of Staphylococcus pseudintermedius isolates.

ABSTRACT:

Background

Staphylococcus pseudintermedius (SP) is the major pathogenic species of dogs involved in a wide variety of skin and soft tissue infections. The accessory gene regulator (agr) locus of Staphylococcus aureus has been extensively studied, and it influences the expression of many virulence genes. It encodes a two-component signal transduction system that leads to down-regulation of surface proteins and up-regulation of secreted proteins during in vitro growth of S. aureus. The objective of this study was to detect and sequence analyzing the AgrA, B, and D of SP isolated from canine skin infections.

Materials and methods

In this study, we have isolated and identified SP from canine pyoderma and otitis cases by polymerase chain reaction (PCR) and confirmed by PCR-restri

SUBMITTER: Chitra MA 

PROVIDER: S-EPMC4774685 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature