Development and evaluation of loop-mediated isothermal amplification assay for rapid detection of Capripoxvirus.

ABSTRACT:

Aim

The present study was undertaken to develop a nucleic acid-based diagnostic assay loop-mediated isothermal amplification assay (LAMP) targeting highly conserved genomic regions of Capripoxvirus (CaPVs) and its comparative evaluation with real-time polymerase chain reaction (PCR).

Material and methods

Lyophilized vaccine strain of sheeppox virus (SPPV) was used for optimization of LAMP assay. The LAMP assay was designed using envelope immunogenic protein (P32) coding gene targeting highly conserved genomic regions of CaPV responsible for causing sheep pox, goat pox, and lumpy skin disease in sheep, goat and cattle respectively. Serial tenfold dilution of SPPV recombinant plasmid DNA was used for a calculating limit of detection. Analytical sensitivity and specificity were

SUBMITTER: Batra K 

PROVIDER: S-EPMC4774739 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature