Single nucleotide markers of D-loop for identification of Indian wild pig (Sus scrofa cristatus).

ABSTRACT:

Aim

Partial fragment of D-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (SNP) markers in the region. The paper also reappraises SNP markers from two fragments of cytochrome b gene and a fragment 12S rRNA gene distinguishing the Indian wild pig from other pig species of the world.

Materials and methods

Deoxyribonucleic acid (DNA) was isolated from 14 and 12 tissue samples of Indian wild and domestic pigs, respectively, collected from Central India for characterization of the D-loop DNA sequences using universal primers. The sequences obtained were aligned along with the retrieved sequences to analyze species-specific SNP

SUBMITTER: Srivastava GK 

PROVIDER: S-EPMC4774806 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature