Biological and molecular characterization of classical swine fever challenge virus from India.

ABSTRACT:

Aim

The aim of this study was biological and molecular characterization of classical swine fever (CSF) challenge virus from India.

Materials and methods

CSF challenge virus maintained at Division of Biological standardization was experimentally infected to two seronegative piglets. The biological characterization was done by clinical sign and symptoms along with postmortem findings. For molecular characterization 5'-nontranslated region, E2 and NS5B regions were amplified by reverse transcription polymerase chain reaction and sequenced. The sequences were compared with that of reference strains and the local field isolates to establish a phylogenetic relation.

Results

The virus produced symptoms of acute disease in the piglets with typical post-mortem lesions. Phyloge

SUBMITTER: Kumar P 

PROVIDER: S-EPMC4774839 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature