Performance of Different Analytical Software Packages in Quantification of DNA Methylation by Pyrosequencing.

ABSTRACT:

Background

Pyrosequencing has emerged as an alternative method of nucleic acid sequencing, well suited for many applications which aim to characterize single nucleotide polymorphisms, mutations, microbial types and CpG methylation in the target DNA. The commercially available pyrosequencing systems can harbor two different types of software which allow analysis in AQ or CpG mode, respectively, both widely employed for DNA methylation analysis.

Objective

Aim of the study was to assess the performance for DNA methylation analysis at CpG sites of the two pyrosequencing software which allow analysis in AQ or CpG mode, respectively. Despite CpG mode having been specifically generated for CpG methylation quantification, many investigations on this topic have been carried out with

SUBMITTER: Grasso C 

PROVIDER: S-EPMC4775062 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature